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ACS Nano. 2017 Nov 28;11(11):11041-11046. doi: 10.1021/acsnano.7b05083. Epub 2017 Oct 25.

Integrated Kidney Exosome Analysis for the Detection of Kidney Transplant Rejection.

Author information

1
Transplantation Research Center, Renal Division, Brigham and Women's Hospital, Harvard Medical School , Boston, Massachusetts 02115, United States.
2
Experimental Therapeutics and Molecular Imaging Laboratory and Department of Neurology, Neuro-Oncology Division, Massachusetts General Hospital , Boston, Massachusetts 02129, United States.
3
Department of Systems Biology, Harvard Medical School , Boston, Massachusetts 02115, United States.

Abstract

Kidney transplant patients require life-long surveillance to detect allograft rejection. Repeated biopsy, albeit the clinical gold standard, is an invasive procedure with the risk of complications and comparatively high cost. Conversely, serum creatinine or urinary proteins are noninvasive alternatives but are late markers with low specificity. We report a urine-based platform to detect kidney transplant rejection. Termed iKEA (integrated kidney exosome analysis), the approach detects extracellular vesicles (EVs) released by immune cells into urine; we reasoned that T cells, attacking kidney allografts, would shed EVs, which in turn can be used as a surrogate marker for inflammation. We optimized iKEA to detect T-cell-derived EVs and implemented a portable sensing system. When applied to clinical urine samples, iKEA revealed high level of CD3-positive EVs in kidney rejection patients and achieved high detection accuracy (91.1%). Fast, noninvasive, and cost-effective, iKEA could offer new opportunities in managing transplant recipients, perhaps even in a home setting.

KEYWORDS:

acute cellular rejection; biosensor; kidney transplant; proteomics; urine exosomes

PMID:
29053921
PMCID:
PMC6237084
DOI:
10.1021/acsnano.7b05083
[Indexed for MEDLINE]
Free PMC Article

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