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Protoplasma. 2018 Mar;255(2):643-654. doi: 10.1007/s00709-017-1172-2. Epub 2017 Oct 19.

Genome-wide analysis and expression profiling of PP2C clade D under saline and alkali stresses in wild soybean and Arabidopsis.

Author information

1
Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
2
Department of chemistry and molecular biology, University of Gothenburg, S-413 90, Gothenburg, Sweden.
3
College of Life Science, Qingdao Agricultural University, Qingdao, People's Republic of China.
4
Crop Stress Molecular Biology Laboratory, Heilongjiang Bayi Agricultural University, Daqing, People's Republic of China.
5
Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, 150030, People's Republic of China. ymzhu2001@neau.edu.cn.

Abstract

Protein phosphatase 2Cs (PP2Cs) belong to the largest protein phosphatase family in plants. Some members have been described as being negative modulators of plant growth and development, as well as responses to hormones and environmental stimuli. However, little is known about the members of PP2C clade D, which may be involved in the regulation of signaling pathways, especially in response to saline and alkali stresses. Here, we identified 13 PP2C orthologs from the wild soybean (Glycine soja) genome. We examined the sequence characteristics, chromosome locations and duplications, gene structures, and promoter cis-elements of the PP2C clade D genes in Arabidopsis and wild soybean. Our results showed that GsPP2C clade D (GsAPD) genes exhibit more gene duplications than AtPP2C clade D genes. Plant hormone and abiotic stress-responsive elements were identified in the promoter regions of most PP2C genes. Moreover, we investigated their expression patterns in roots, stems, and leaves. Quantitative real-time PCR analyses revealed that the expression levels of representative GsPP2C and AtPP2C clade D genes were significantly influenced by alkali and salt stresses, suggesting that these genes might be associated with or directly involved in the relevant stress signaling pathways. Our results established a foundation for further functional characterization of PP2C clade D genes in the future.

KEYWORDS:

Alkali; Arabidopsis; PP2C clade D; Salt; Wild soybean

PMID:
29052008
DOI:
10.1007/s00709-017-1172-2
[Indexed for MEDLINE]

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