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PLoS One. 2017 Oct 19;12(10):e0185790. doi: 10.1371/journal.pone.0185790. eCollection 2017.

Protein structural disorder of the envelope V3 loop contributes to the switch in human immunodeficiency virus type 1 cell tropism.

Author information

1
Evolution & Genomic Sciences, School of Biological Sciences, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom.
2
Department of Genetics, University of Cambridge, Cambridge, United Kingdom.
3
Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.
4
Department of Biology, University of Nevada, Reno, Nevada, United States of America.
5
MRC-University of Glasgow Centre for Virus Research, Garscube Campus, Glasgow, United Kingdom.

Abstract

Human immunodeficiency virus type 1 (HIV-1) envelope gp120 is partly an intrinsically disordered (unstructured/disordered) protein as it contains regions that do not fold into well-defined protein structures. These disordered regions play important roles in HIV's life cycle, particularly, V3 loop-dependent cell entry, which determines how the virus uses two coreceptors on immune cells, the chemokine receptors CCR5 (R5), CXCR4 (X4) or both (R5X4 virus). Most infecting HIV-1 variants utilise CCR5, while a switch to CXCR4-use occurs in the majority of infections. Why does this 'rewiring' event occur in HIV-1 infected patients? As changes in the charge of the V3 loop are associated with this receptor switch and it has been suggested that charged residues promote structure disorder, we hypothesise that the intrinsic disorder of the V3 loop is permissive to sequence variation thus contributing to the switch in cell tropism. To test this we use three independent data sets of gp120 to analyse V3 loop disorder. We find that the V3 loop of X4 virus has significantly higher intrinsic disorder tendency than R5 and R5X4 virus, while R5X4 virus has the lowest. These results indicate that structural disorder plays an important role in HIV-1 cell tropism and CXCR4 binding. We discuss the potential evolutionary mechanisms leading to the fixation of disorder promoting mutations and the adaptive potential of protein structural disorder in viral host adaptation.

PMID:
29049306
PMCID:
PMC5648111
DOI:
10.1371/journal.pone.0185790
[Indexed for MEDLINE]
Free PMC Article

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