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Front Cell Infect Microbiol. 2017 Oct 4;7:430. doi: 10.3389/fcimb.2017.00430. eCollection 2017.

Inactive trans-Sialidase Expression in iTS-null Trypanosoma cruzi Generates Virulent Trypomastigotes.

Author information

1
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, Buenos Aires, Argentina.
2
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
3
Instituto Nacional de Parasitología "Dr. Mario Fatala Chabén", Administración Nacional de Laboratorio e Institutos de Salud, "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.

Abstract

Disclosing virulence factors from pathogens is required to better understand the pathogenic mechanisms involved in their interaction with the host. In the case of Trypanosoma cruzi several molecules are associated with virulence. Among them, the trans-sialidase (TS) has arisen as one of particular relevance due to its effect on the immune system and involvement in the interaction/invasion of the host cells. The presence of conserved genes encoding for an inactive TS (iTS) isoform is puzzlingly restricted to the genome of parasites from the Discrete Typing Units TcII, TcV, and TcVI, which include highly virulent strains. Previous in vitro results using recombinant iTS support that this isoform could play a different or complementary pathogenic role to that of the enzymatically active protein. However, direct evidence involving iTS in in vivo pathogenesis and invasion is still lacking. Here we faced this challenge by transfecting iTS-null parasites with a recombinant gene that allowed us to follow its expression and association with pathological events. We found that iTS expression improves parasite invasion of host cells and increases their in vivo virulence for mice as shown by histopathologic findings in heart and skeletal muscle.

KEYWORDS:

Discrete Typing Units; Trypanosoma cruzi virulence; inactive trans-sialidase; pathogenesis; virulence factors

PMID:
29046868
PMCID:
PMC5632715
DOI:
10.3389/fcimb.2017.00430
[Indexed for MEDLINE]
Free PMC Article

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