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Plant J. 2017 Dec;92(6):1132-1142. doi: 10.1111/tpj.13749. Epub 2017 Nov 20.

A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association.

Author information

1
'Bioimage and Bioinformatics' Platform, Institut de Biologie Moléculaire des Plantes, UPR 2357 du CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, Strasbourg Cedex, 67084, France.
2
'Metabolism and Trafficking of RNA Within the Plant Cell' Team, Institut de Biologie Moléculaire des Plantes, UPR 2357 du CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, Strasbourg Cedex, 67084, France.

Abstract

Intracellular sorting of mRNAs is an essential process for regulating gene expression and protein localization. Most mitochondrial proteins are nuclear-encoded and imported into the mitochondria through post-translational or co-translational processes. In the latter case, mRNAs are found to be enriched in the vicinity of mitochondria. A genome-scale analysis of mRNAs associated with mitochondria has been performed to determine plant cytosolic mRNAs targeted to the mitochondrial surface. Many messengers encoding mitochondrial proteins were found associated with mitochondria. These mRNAs correspond to particular functions and complexes, such as respiration or mitoribosomes, which indicates a coordinated control of mRNA localization within metabolic pathways. In addition, upstream AUGs in 5' untranslated regions (UTRs), which modulate the translation efficiency of downstream sequences, were found to negatively affect the association of mRNAs with mitochondria. A mutational approach coupled with in vivo mRNA visualization confirmed this observation. Moreover, this technique allowed the identification of 3'-UTRs as another essential element for mRNA localization at the mitochondrial surface. Therefore, this work offers new insights into the mechanism, function and regulation of the association of cytosolic mRNAs with plant mitochondria.

KEYWORDS:

uAUG ; uORF ; 3' UTR; mRNA localization; respiration; ribosome; translation

PMID:
29044717
DOI:
10.1111/tpj.13749
[Indexed for MEDLINE]
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