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Methods Mol Biol. 2018;1672:1-9. doi: 10.1007/978-1-4939-7306-4_1.

The A-Like Faker Assay for Measuring Yeast Chromosome III Stability.

Author information

1
Terry Fox Laboratory, BC Cancer Agency, 675 W. 10th Ave, Vancouver, Canada.
2
Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, Canada.
3
Terry Fox Laboratory, BC Cancer Agency, 675 W. 10th Ave, Vancouver, Canada. pstirling@bccrc.ca.
4
Department of Medical Genetics, University of British Columbia, Vancouver, Canada. pstirling@bccrc.ca.

Abstract

The ability to rapidly assess chromosome instability (CIN) has enabled profiling of most yeast genes for potential effects on genome stability. The A-like faker (ALF) assay is one of several qualitative and quantitative marker loss assays that indirectly measure loss or conversion of genetic material using a counterselection step. The ALF assay relies on the ability to count spurious mating events that occur upon loss of the MATĪ± locus of haploid Saccharomyces cerevisiae strains. Here, we describe the deployment of the ALF assay for both rapid and simple qualitative, and more in-depth quantitative analysis allowing determination of absolute ALF frequencies.

KEYWORDS:

A-like faker; Aneuploidy; Chromosome instability; Gene conversion; Genome instability; Gross chromosomal rearrangement; Marker loss

PMID:
29043612
DOI:
10.1007/978-1-4939-7306-4_1
[Indexed for MEDLINE]

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