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J Struct Biol. 2017 Nov;200(2):124-127. doi: 10.1016/j.jsb.2017.10.004. Epub 2017 Oct 16.

Online Raman spectroscopy for structural biology on beamline ID29 of the ESRF.

Author information

1
European Synchrotron Radiation Facility (ESRF), F-38043 Grenoble, France. Electronic address: david.von_stetten@esrf.fr.
2
European Synchrotron Radiation Facility (ESRF), F-38043 Grenoble, France.
3
Randall Centre for Cell and Molecular Biophysics, Faculty of Life Sciences and Medicine, King's College London, SE1 1UL London, UK.
4
6tec, 745 route de Grenoble, F-38260 La Frette, France.
5
European Synchrotron Radiation Facility (ESRF), F-38043 Grenoble, France. Electronic address: daniele.de_sanctis@esrf.fr.
6
European Synchrotron Radiation Facility (ESRF), F-38043 Grenoble, France; Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale), F-38000 Grenoble, France. Electronic address: antoine.royant@ibs.fr.

Abstract

Raman spectroscopy can probe the structure and conformations of specific chemical groups within proteins and may thus be used as a technique complementary to X-ray crystallography. This combined approach can be decisive in resolving ambiguities in the interpretation of enzymatic or X-ray induced processes. Here, we present an online Raman setup developed at the European Synchrotron that allows for interleaved Raman spectra acquisition and X-ray diffraction measurements with fast probe exchange and simple alignment while maintaining a high sensitivity over the entire spectral range. This device has been recently employed in the study of a covalent intermediate in the O2-dependent breakdown of uric acid by the cofactor-free enzyme urate oxidase and to monitor its decay induced by X-ray exposure.

KEYWORDS:

Diffraction-complementary technique; Kinetic crystallography; Macromolecular crystallography; Radiation damage; Raman spectroscopy

PMID:
29042242
DOI:
10.1016/j.jsb.2017.10.004
[Indexed for MEDLINE]

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