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Vector Borne Zoonotic Dis. 2018 Jan;18(1):2-13. doi: 10.1089/vbz.2017.2121. Epub 2017 Oct 17.

Recommendations for Laboratory Containment and Management of Gene Drive Systems in Arthropods.

Author information

1
1 Entomology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia .
2
2 Life Sciences, Imperial College London , Ascot, United Kingdom .
3
3 Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo , Sao Paulo, Brazil .
4
4 National Institute of Science and Technology in Molecular Entomology , National Council of Scientific and Technological Development (INCT-EM/CNPq), Rio de Janeiro, Brazil .
5
5 CSIRO , Brisbane, Australia .
6
6 USDA-ARS, Center for Medical, Agricultural, and Veterinary Entomology , Gainesville, Florida.
7
7 CSIRO , Hobart, Australia .
8
8 Divisions of Biostatistics and Epidemiology, School of Public Health, University of California , Berkeley, California.
9
9 Florida Medical Entomology Laboratory, Department of Entomology and Nematology, University of Florida , Vero Beach, Florida.
10
10 Department of Entomology, Texas A&M University , College Station, Texas.

Abstract

Versatile molecular tools for creating driving transgenes and other invasive genetic factors present regulatory, ethical, and environmental challenges that should be addressed to ensure their safe use. In this article, we discuss driving transgenes and invasive genetic factors that can potentially spread after their introduction into a small proportion of individuals in a population. The potential of invasive genetic factors to increase their number in natural populations presents challenges that require additional safety measures not provided by previous recommendations regarding accidental release of arthropods. In addition to providing physical containment, invasive genetic factors require greater attention to strain management, including their distribution and identity confirmation. In this study, we focus on insects containing such factors with recommendations for investigators who are creating them, institutional biosafety committees charged with ensuring safety, funding agencies providing support, those managing insectaries handling these materials who are responsible for containment, and other persons who will be receiving insects-transgenic or not-from these facilities. We give specific examples of efforts to modify mosquitoes for mosquito-borne disease control, but similar considerations are relevant to other arthropods that are important to human health, the environment, and agriculture.

KEYWORDS:

biosafety; gene drive; mosquitoes; transgenic organism

PMID:
29040058
PMCID:
PMC5846571
DOI:
10.1089/vbz.2017.2121
[Indexed for MEDLINE]
Free PMC Article

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