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Methods Mol Biol. 2018;1682:37-47. doi: 10.1007/978-1-4939-7352-1_4.

Elemental Analysis in Biological Matrices Using ICP-MS.

Author information

1
Cancer Research Technology Program, Nanotechnology Characterization Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, P.O. Box B, Frederick, MD, 21702, USA.
2
Cancer Research Technology Program, Nanotechnology Characterization Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, P.O. Box B, Frederick, MD, 21702, USA. Jeffrey.Clogston@fnlcr.nih.gov.

Abstract

The increasing exploration of metallic nanoparticles for use as cancer therapeutic agents necessitates a sensitive technique to track the clearance and distribution of the material once introduced into a living system. Inductively coupled plasma mass spectrometry (ICP-MS) provides a sensitive and selective tool for tracking the distribution of metal components from these nanotherapeutics. This chapter presents a standardized method for processing biological matrices, ensuring complete homogenization of tissues, and outlines the preparation of appropriate standards and controls. The method described herein utilized gold nanoparticle-treated samples; however, the method can easily be applied to the analysis of other metals.

KEYWORDS:

Biodistribution; Biological matrices; Blood; Homogenization; ICP-MS; Nanoparticles

PMID:
29039091
DOI:
10.1007/978-1-4939-7352-1_4
[Indexed for MEDLINE]

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