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Methods Mol Biol. 2018;1682:19-22. doi: 10.1007/978-1-4939-7352-1_2.

Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test.

Author information

1
Cancer Research Technology Program, Nanotechnology Characterization Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, P.O. Box B, Frederick, MD, 21702, USA.
2
Cancer Research Technology Program, Nanotechnology Characterization Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, P.O. Box B, Frederick, MD, 21702, USA. marina@mail.nih.gov.

Abstract

Bacterial contamination can confound the results of in vitro and in vivo preclinical tests. This protocol describes a procedure for detection of microbial contamination in nanotechnology-based formulations. Nanoparticle samples and controls are spread on the surface of agar and growth of bacterial colonies is monitored after 72 h of incubation. The intended purpose of this assay is to avoid introduction of microbial contamination into in vitro cell cultures and in vivo animal studies utilizing the test nanomaterial. This assay is not intended to certify the material as sterile.

KEYWORDS:

Bacteria; Contamination; Mold; Nanoparticles; Yeast

PMID:
29039089
DOI:
10.1007/978-1-4939-7352-1_2
[Indexed for MEDLINE]

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