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Sci Rep. 2017 Oct 16;7(1):13290. doi: 10.1038/s41598-017-13513-x.

Involvement of Ubiquitin-Conjugating Enzyme (E2 Gene Family) in Ripening Process and Response to Cold and Heat Stress of Vitis vinifera.

Gao Y1,2,3, Wang Y2,3, Xin H1, Li S2, Liang Z4,5.

Author information

1
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, PR China.
2
Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resource, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, PR China.
3
University of Chinese Academy of Sciences, Beijing, 100049, PR China.
4
Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resource, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, PR China. ZL249@ibcas.ac.cn.
5
Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, 430074, PR China. ZL249@ibcas.ac.cn.

Abstract

Ubiquitin-conjugating (UBC) E2 enzyme plays crucial roles in plant growth and development. Limited information can describe the function of UBC enzyme E2 in grapes. A total of 43 UBC enzyme E2 genes with conserved UBC domain were identified in grapes. These genes were divided into five groups based on phylogenetic tree with tomatoes. Sequence analyses indicated that VvUBCs in the same group possessed similar gene structures and conserved motifs. Gene distribution in chromosomes was uneven, and gene duplication existed in 36 VvUBCs. Transcriptome and qRT-PCR analysis indicated that most VvUBCs are involved in ripening and post-harvest stage, and feature functional roles in grape organs. According to the transcriptome and qRT-PCR results, seven and six VvUBCs in grape responded to cold and heat stress, respectively, whereas no remarkable VvUBCs change was noted under salt or water-deficit stress. This study provides new insights to physiological and developmental roles of these enzymes and regulation mechanism of E2 genes in grapes.

PMID:
29038452
PMCID:
PMC5643510
DOI:
10.1038/s41598-017-13513-x
[Indexed for MEDLINE]
Free PMC Article

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