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Biochem Biophys Res Commun. 2017 Dec 9;494(1-2):181-187. doi: 10.1016/j.bbrc.2017.10.059. Epub 2017 Oct 14.

One-step construction of a molybdenum disulfide/multi-walled carbon nanotubes/polypyrrole nanocomposite biosensor for the ex-vivo detection of dopamine in mouse brain tissue.

Author information

1
Graduate Department of Chemical Materials, Pusan National University, Busan, 46241, Republic of Korea.
2
College of Pharmacy, Molecular Inflammation Research Center for Aging Intervention, Pusan National University, Busan, 46241, Republic of Korea.
3
Department of Chemistry, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University, Busan, 46241, Republic of Korea.
4
School of Pharmacy, Sungkyunkwan University, Suwon, 16419, Republic of Korea.
5
College of Pharmacy, Molecular Inflammation Research Center for Aging Intervention, Pusan National University, Busan, 46241, Republic of Korea. Electronic address: neuron@pusan.ac.kr.
6
Institute of BioPhysio Sensor Technology, Pusan National University, Busan, 46241, Republic of Korea. Electronic address: s.c.chang@pusan.ac.kr.

Abstract

We developed a new strategy for construction of a biosensor for the neurotransmitter dopamine. The biosensor was constructed by one-step electrochemical deposition of a nanocomposite in aqueous solution at pH 7.0, consisting of molybdenum disulfide, multi-walled carbon nanotubes, and polypyrrole. A series of analytical methods was performed to investigate the surface characteristics and the improved electrocatalytic effect of the nanocomposite, including cyclic voltammetry, electrochemical impedance spectroscopy, field-emission scanning electron microscopy, atomic force microscopy, and Raman spectroscopy. The constructed biosensor showed high sensitivity (1.130 μAμM-1cm-2) with a dynamic linearity range of 25-1000 nM and a detection limit of 10 nM. Additionally, the designed sensor exhibited strong anti-interference ability and satisfactory reproducibility. The practical application of the sensor was manifested for the ex vivo determination of dopamine neurotransmitters using brain tissue samples of a mouse Parkinson's disease model.

KEYWORDS:

Dopamine; Ex vivo sensor; Molybdenum disulfide; Mouse Parkinson's disease model; One-step electrochemical deposition

PMID:
29037811
DOI:
10.1016/j.bbrc.2017.10.059
[Indexed for MEDLINE]

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