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Food Chem. 2018 Mar 1;242:497-504. doi: 10.1016/j.foodchem.2017.09.091. Epub 2017 Sep 19.

Mass spectrometry-based phytochemical screening for hypoglycemic activity of Fagioli di Sarconi beans (Phaseolus vulgaris L.).

Author information

1
Scuola di Ingegneria, Università degli Studi della Basilicata, via dell'Ateneo Lucano, 10, 85100 Potenza, Italy.
2
Dipartimento di Scienze, Università degli Studi della Basilicata, via dell'Ateneo Lucano, 10, 85100 Potenza, Italy. Electronic address: giuliana.bianco@unibas.it.
3
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via E. Orabona, 4, 70126 Bari, Italy.
4
Helmholtz Zentrum Muenchen, Analytical BioGeoChemistry, Ingolstaedter Landstr. 1, D-85764 Neuherberg, Germany; Technische Universität Muenchen, Chair of Analytical Food Chemistry, Alte Akademie 10, D-85354 Weihenstephan/Freising, Germany.
5
Dipartimento di Scienze, Università degli Studi della Basilicata, via dell'Ateneo Lucano, 10, 85100 Potenza, Italy.
6
Helmholtz Zentrum Muenchen, Analytical BioGeoChemistry, Ingolstaedter Landstr. 1, D-85764 Neuherberg, Germany.

Abstract

The present study deals with the evaluation of antidiabetic activities of Fagioli di Sarconi beans (Phaseolus vulgaris), including 21 ecotypes protected by the European Union with the mark PGI (i.e., Protected Geographical Indication), and cultivated in Basilicata (southern Italy). For this purpose, α-glucosidase and α-amylase assays were assessed; among all bean ecotypes, the tight green seed colour of Verdolino extracts exhibited the highest α-glucosidase and α-amylase inhibitory activity with IC50=1.1±0.1μg/ml and IC50=19.3±1.1μg/ml, respectively. Phytochemical compound screening of all Fagioli di Sarconi beans performed by flow injection-electrospray ionization-ultrahigh resolution mass spectrometry (uHRMS) and based on the calculation of elemental formulas from accurate m/z values, was helpful to annotate specific compounds, such as alkaloids, saponins, flavonoids, and terpenoids, which are most likely responsible for their biological activity.

KEYWORDS:

Antidiabetic activity; Fagioli di Sarconi beans; High-resolution mass spectrometry; Phaseouls vulgaris L.; Phytochemical profile; α-Amylase; α-Glucosidase

PMID:
29037720
DOI:
10.1016/j.foodchem.2017.09.091
[Indexed for MEDLINE]

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