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Rev Bras Reumatol Engl Ed. 2017 Sep - Oct;57(5):385-391. doi: 10.1016/j.rbre.2016.09.007. Epub 2016 Oct 18.

Correlation between cellular expression of complement regulatory proteins with depletion and repopulation of B-lymphocytes in peripheral blood of patients with rheumatoid arthritis treated with rituximab.

[Article in English, Portuguese]

Author information

1
Hospital de Clínicas de Porto Alegre, Serviço de Reumatologia, Porto Alegre, RS, Brazil. Electronic address: daniviecceli@gmail.com.
2
Hospital de Clínicas de Porto Alegre, Serviço de Patologia Clínica, Porto Alegre, RS, Brazil.
3
Hospital de Clínicas de Porto Alegre, Serviço de Reumatologia, Porto Alegre, RS, Brazil.

Abstract

OBJECTIVES:

To correlate the basal expression of complement regulatory proteins (CRPs) CD55, CD59, CD35, and CD46 in B-lymphocytes from the peripheral blood of a cohort of 10 patients with rheumatoid arthritis (RA) initiating treatment with rituximab (RTX) with depletion and time repopulation of such cells.

METHODS:

Ten patients with RA received two infusions of 1g of RTX with an interval of 14 days. Immunophenotypic analysis for the detection of CD55, CD59, CD35, and CD46 on B-lymphocytes was carried out immediately before the first infusion. The population of B-lymphocytes was analyzed by means of basal CD19 expression and after 1, 2, and 6 months after the infusion of RTX, and then quarterly until clinical relapse. Depletion of B-lymphocytes in peripheral blood was defined as a CD19 expression <0.005×109/L.

RESULTS:

Ten women with a median of 49 years and a baseline DAS28=5.6 were evaluated; 9 were seropositive for rheumatoid factor. Five patients showed a repopulation of B-lymphocytes after 2 months, and the other five after 6 months. There was a correlation between the basal expression of CD46 and the time of repopulation (correlation coefficient=-0.733, p=0.0016). A similar trend was observed with CD35, but without statistical significance (correction coefficient=-0.522, p=0.12).

CONCLUSION:

The increased CD46 expression was predictive of a faster repopulation of B-lymphocytes in patients treated with RTX. Studies involving a larger number of patients will be needed to confirm the utility of basal expression of CRPs as a predictor of clinical response.

KEYWORDS:

Artrite reumatoide; Biomarcadores; Biomarkers; Complement regulatory proteins; Proteínas reguladoras do complemento; Rheumatoid arthritis; Rituximab; Rituximabe

PMID:
29037309
DOI:
10.1016/j.rbre.2016.09.007
[Indexed for MEDLINE]
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