Format

Send to

Choose Destination
Nucleic Acids Res. 2018 Jan 4;46(D1):D121-D126. doi: 10.1093/nar/gkx875.

ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization.

Sang J1,2,3, Wang Z3,4, Li M1,2,3, Cao J1,2,3, Niu G1,2,3, Xia L1,2,3, Zou D1,2, Wang F1,2, Xu X1,2, Han X5, Fan J6, Yang Y7, Zuo W7, Zhang Y1,2,3, Zhao W1,3, Bao Y1,2,3, Xiao J1,2,3,8, Hu S2,3,8, Hao L1,2, Zhang Z1,2,3,8.

Author information

1
BIG Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
2
CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
3
University of Chinese Academy of Sciences, Beijing 100049, China.
4
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
5
Key Laboratory of Tree Genomics, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
6
School of Ocean, Yantai University, Yantai 264005, China.
7
School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.
8
Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai 200438, China.

Abstract

Real-time quantitative PCR (RT-qPCR) has become a widely used method for accurate expression profiling of targeted mRNA and ncRNA. Selection of appropriate internal control genes for RT-qPCR normalization is an elementary prerequisite for reliable expression measurement. Here, we present ICG (http://icg.big.ac.cn), a wiki-driven knowledgebase for community curation of experimentally validated internal control genes as well as their associated experimental conditions. Unlike extant related databases that focus on qPCR primers in model organisms (mainly human and mouse), ICG features harnessing collective intelligence in community integration of internal control genes for a variety of species. Specifically, it integrates a comprehensive collection of more than 750 internal control genes for 73 animals, 115 plants, 12 fungi and 9 bacteria, and incorporates detailed information on recommended application scenarios corresponding to specific experimental conditions, which, collectively, are of great help for researchers to adopt appropriate internal control genes for their own experiments. Taken together, ICG serves as a publicly editable and open-content encyclopaedia of internal control genes and accordingly bears broad utility for reliable RT-qPCR normalization and gene expression characterization in both model and non-model organisms.

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center