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Biomaterials. 2018 Jan;150:100-111. doi: 10.1016/j.biomaterials.2017.10.016. Epub 2017 Oct 9.

Mechanotransduction of human pluripotent stem cells cultivated on tunable cell-derived extracellular matrix.

Author information

1
Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea.
2
Department of Stem Cell Biology, Konkuk University, School of Medicine, Seoul, 05029, Republic of Korea.
3
Soonchunhyang Institute of Medi-bio Science (SIMS), Institute of Tissue Regeneration, College of Medicine, Soon Chun Hyang University, Cheonan, 31151, South Korea.
4
Department of Stem Cell Biology, Konkuk University, School of Medicine, Seoul, 05029, Republic of Korea. Electronic address: sunghwanmoon@kku.ac.kr.
5
Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea; Biomedical Engineering Major, Korea University of Science and Technology (UST), Daejeon, 34113, Republic of Korea. Electronic address: kpark@kist.re.kr.

Abstract

Cell-derived matrices (CDM) are becoming an attractive alternative to conventional biological scaffolding platforms due to its unique ability to closely recapitulate a native extracellular matrix (ECM) de novo. Although cell-substrate interactions are recognized to be principal in regulating stem cell behavior, very few studies have documented the acclimation of human pluripotent stem cells (hPSCs) on pristine and altered cell-derived matrices. Here, we investigate crosslink-induced mechanotransduction of hPSCs cultivated on decellularized fibroblast-derived matrices (FDM) to explore cell adhesion, growth, migration, and pluripotency in various biological landscapes. The results showed either substrate-mediated induction or inhibition of the Epithelial-Mesenchymal-Transition (EMT) program, strongly suggesting that FDM stiffness can be a dominant factor in mediating hPSC plasticity. We further propose an optimal FDM substratum intended for long-term hPSC cultivation in a feeder-free niche-like microenvironment. This study carries significant implications for hPSC cultivation and encourages more in-depth studies towards the fundamentals of hPSC-CDM interactions.

[Indexed for MEDLINE]

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