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Stem Cell Res. 2017 Oct;24:16-20. doi: 10.1016/j.scr.2017.08.006. Epub 2017 Aug 8.

Generation and characterization of a human induced pluripotent stem (iPS) cell line derived from an acute myeloid leukemia patient evolving from primary myelofibrosis carrying the CALR 52bp deletion and the ASXL1 p.R693X mutation.

Author information

1
Molecular Carcinogenesis Program, Research Coordination, Brazilian National Cancer Institute (INCA), Rua André Cavalcante 37, Rio de Janeiro 20230-240, Brazil.
2
D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro 30, Rio de Janeiro 22281-100, Brazil.
3
Laboratory of Molecular Biology, Bone Marrow Transplant Center (CEMO), Brazilian National Cancer Institute (INCA), Praça Cruz Vermelha, 23, Rio de Janeiro 20230-130, Brazil.
4
Pedro Ernesto University Hospital (HUPE), University of the State of Rio de Janeiro (UERJ), Boulevard 28 de Setembro, 77, Rio de Janeiro 20551-030, Brazil.
5
Antônio Pedro University Hospital (HUAP), Fluminense Federal University (UFF), Rua Marques de Paraná, 303, Niterói 24033-900, Brazil.
6
D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro 30, Rio de Janeiro 22281-100, Brazil; Institute of Biomedical Sciences, Federal University of Rio de Janeiro (UFRJ), Avenida Carlos Chagas 373, Rio de Janeiro 21941-590, Brazil.
7
Laboratory of Molecular Biology, Bone Marrow Transplant Center (CEMO), Brazilian National Cancer Institute (INCA), Praça Cruz Vermelha, 23, Rio de Janeiro 20230-130, Brazil. Electronic address: barbara.montemor@inca.gov.br.
8
Molecular Carcinogenesis Program, Research Coordination, Brazilian National Cancer Institute (INCA), Rua André Cavalcante 37, Rio de Janeiro 20230-240, Brazil; FIOCRUZ - Oswaldo Cruz Foundation Institute, Avenida Brasil 4365 - Manguinhos, Rio de Janeiro 21040-360, Brazil. Electronic address: mbonamino@inca.gov.br.

Abstract

Peripheral blood sample was donated by a 61years old female patient diagnosed with acute myeloid leukemia secondary to a primary myelofibrosis harboring the 52-bp deletion in the CALR gene (c.1092_1143del, p.L367fs*46) and the R693X mutation in the ASXL1 gene (c.2077C>T, p.R693X). CD34+ cells were isolated from the sample and subjected to the reprogramming procedure by using the Sendai virus carrying the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc. iPS colonies generated retained the original mutations and displayed all the features of bona fide iPS cells.

PMID:
29034885
DOI:
10.1016/j.scr.2017.08.006
[Indexed for MEDLINE]
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