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Stem Cell Res. 2017 Oct;24:148-150. doi: 10.1016/j.scr.2017.02.009. Epub 2017 Feb 24.

Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a modified RNA system.

Author information

1
Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, South Korea.
2
Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, South Korea. Electronic address: skkoo@nih.go.kr.

Abstract

We generated human induced pluripotent stem cells (KSCBi002-B and KSCBi002-B-1) from the dermal fibroblasts of a donor using a modified RNA-based gene delivery method. According to GTG-banding analysis, the generated KSCBi002-B line has a cytogenetic abnormality (46,XY, t(1;4)(q21;q25)) that is distinct from that of the donor, whereas KSCBi002-B-1 has a normal karyotype (46,XY). These cell lines can be useful as a model for characterizing the hiPSCs generated by a non-viral and non-integrative system, or as a chromosomal balanced translocation model. These two cell lines are registered and available from the National Stem Cell Bank, Korea National Institute of Health.

PMID:
29034882
DOI:
10.1016/j.scr.2017.02.009
[Indexed for MEDLINE]
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