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Front Plant Sci. 2017 Sep 25;8:1642. doi: 10.3389/fpls.2017.01642. eCollection 2017.

Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii.

Author information

1
Instituto Nacional de SemillasCanelones, Uruguay.
2
Unidad de Biotecnología, Instituto Nacional de Investigación AgropecuariaCanelones, Uruguay.
3
Departamento de Biociencias, Facultad de Química, Universidad de la RepúblicaMontevideo, Uruguay.
4
Departamento de Producción Vegetal, Centro Regional Sur, Facultad de Agronomía, Universidad de la RepúblicaCanelones, Uruguay.
5
The Sainsbury Laboratory, Norwich Research ParkNorwich, United Kingdom.
6
Programa de Producción Hortícola, Instituto Nacional de Investigación AgropecuariaCanelones, Uruguay.

Abstract

Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato.

KEYWORDS:

EFR; Ralstonia solanacearum; Solanum commersonii; Solanum tuberosum; bacterial wilt; pattern recognition receptor; quantitative resistance

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