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J Endod. 2017 Dec;43(12):2041-2047. doi: 10.1016/j.joen.2017.07.018. Epub 2017 Oct 9.

Bortezomib Facilitates Reparative Dentin Formation after Pulp Access Cavity Preparation in Mouse Molar.

Author information

1
Department of Oral Medicine, School of Dentistry, Kyungpook National University, Daegu, Korea.
2
Department of Biochemistry, School of Dentistry, Institute for Hard Tissue and Bone Regeneration, Kyungpook National University, Daegu, Korea.
3
Department of Dental Hygiene, Kyungpook National University, Daegu, Korea.
4
Department of Dental Hygiene, Gachon University College of Health Science, Incheon, Seoul, Korea.
5
Department of Oral and Maxillofacial Radiology, Kyungpook National University, Daegu, Korea.
6
Department of Dental Materials, School of Dentistry, Kyungpook National University, Daegu, Korea.
7
Department of Biochemistry, School of Dentistry, Institute for Hard Tissue and Bone Regeneration, Kyungpook National University, Daegu, Korea. Electronic address: jykim91@knu.ac.kr.
8
Department of Conservative Dentistry, School of Dentistry, Institute for Hard Tissue and Bone Regeneration, Kyungpook National University, Daegu, Korea. Electronic address: endoking@knu.ac.kr.

Abstract

INTRODUCTION:

The aim of this study was to evaluate in vitro and ex vivo roles of bortezomib, a proteasome inhibitor that binds to the active site of the 26S proteasome, in tertiary dentin formation.

METHODS:

We established pulpal access cavity preparation that was treated with or without bortezomib before direct pulp capping with a calcium hydroxide-based material. We also analyzed bone morphogenetic protein (Bmp)- and Wnt-related signaling molecules using quantitative real-time polymerase chain reaction.

RESULTS:

In the short-term observation period, the bortezomib-treated pulp specimens showed the period-altered immunolocalization patterns of nestin, CD31, and myeloperoxidase, whereas the control specimens did not. The bortezomib-treated group showed a complete dentin bridge with very few irregular tubules after 42 days. The micro-computed tomographic images showed more apparent dentin bridge structures in the treated specimens than were in the controls. Quantitative real-time polymerase chain reaction analysis showed up-regulated Bmp and Wnt.

CONCLUSIONS:

These findings revealed that treatment with 1 μmol/L bortezomib induced reparative dentin formation that facilitated the maintenance of the integrity of the remaining pulpal tissue via early vascularization and regulation of Bmp and Wnt signaling.

KEYWORDS:

Cavity preparation; Wnt signaling; dentin-pulp complex; reparative dentin formation; vascularization

PMID:
29032823
DOI:
10.1016/j.joen.2017.07.018
[Indexed for MEDLINE]

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