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Biosci Biotechnol Biochem. 2017 Dec;81(12):2323-2329. doi: 10.1080/09168451.2017.1385383. Epub 2017 Oct 16.

Titrated extract of Centella asiatica increases hair inductive property through inhibition of STAT signaling pathway in three-dimensional spheroid cultured human dermal papilla cells.

Author information

1
a Department of Cosmetics Engineering , Research Institute for Molecular-Targeted Drugs, Konkuk University , Seoul , Republic of Korea.
2
b Korea Institute for Skin and Clinical Sciences, Gene Cell Pharm Corporation , Seoul , Republic of Korea.
3
c Laboratory of Molecular Oncology , Cheil General Hospital and Women's Healthcare Center, Dankook University College of Medicine , Seoul , Republic of Korea.
4
d Department of Cosmetology, Graduate School of Engineering , Konkuk University , Seoul , Republic of Korea.
5
e Department of Image Industry, Graduate School of Engineering , Konkuk University , Seoul , Republic of Korea.
6
f Department of Dermatology , Konkuk University School of Medicine , Seoul , Republic of Korea.

Abstract

Dermal papilla (DP) is a pivotal part of hair follicle, and the smaller size of the DP is related with the hair loss. In this study, we investigated the effect of titrated extract of Centella asiatica (TECA) on hair growth inductive property on 3D spheroid cultured human DP cells (HDP cells). Significantly increased effect of TECA on cell viability was only shown in 3D sphered HPD cells, not in 2D cultured HDP cells. Also, TECA treatment increased the sphere size of HDP cells. The luciferase activity of STAT reporter genes and the expression of STAT-targeted genes, SOCS1 and SOCS3, were significantly decreased. Also, TECA treatment increased the expression of the hair growth-related signature genes in 3D sphered HDP cells. Furthermore, TECA led to downregulation of the level of phosphorylated STAT proteins in 3D sphered HDP cells. Overall, TECA activates the potential of hair inductive capacity in HDP cells.

KEYWORDS:

STAT; cell culture; dermal papilla cells; hair growth; titrated extract of Centella asiatica

PMID:
29032741
DOI:
10.1080/09168451.2017.1385383
[Indexed for MEDLINE]

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