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J Vet Sci. 2018 Mar 31;19(2):280-289. doi: 10.4142/jvs.2018.19.2.280.

Haemoproteus in barn and collared scops owls from Thailand.

Author information

1
Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University Kamphaeng Saen Campus, Nakorn Pathom 73140, Thailand.
2
Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10903, Thailand.
3
Kasetsart University Raptor Rehabilitation Unit, Faculty of Veterinary Medicine, Kasetsart University Kamphaeng Saen Campus, Nakorn Pathom 73140, Thailand.
4
Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University Kamphaeng Saen Campus, Nakorn Pathom 73140, Thailand.

Abstract

The barn owl (BO) and the collared scops owl (CSO) are common nocturnal raptors throughout Thailand. Blood samples from 23 adult BOs and 14 CSOs were collected and processed for complete blood cell counts and parasite morphological examinations. Two Haemoproteus-positive samples were processed for ultrastructural observation. Polymerase chain reaction (PCR) analysis for a partial cytochrome b gene (cytb) from Haemoproteus was performed in all samples. Haemoproteus presence detected by light microscopy was lower than that detected by PCR (30.4% and 34.8%, respectively, in BO; and 50.0% and 78.6%, respectively, in CSO). Comparative hematology revealed that Haemoproteus-positive BOs had higher mean cell hemoglobin concentration, total leukocyte, absolute heterophil, basophil, and monocyte counts than Haemoproteus-negative BOs, but no significant differences between Haemoproteus-negative and -positive CSOs. Monocyte ultrastructure analysis revealed a role in the elimination of gametocytes. Morphologically, the Haemoproteus in 3 BOs and 6 CSOs were identified as H. noctuae, while that in 1 CSO was identified as H. syrnii. Phylogenetic analysis indicated the Haemoproteus spp. in 8 BOs and 7 CSOs were not closely related to H. noctuae or H. syrnii, and the cytb of 2 CSOs was that of H. syrnii. These results should be useful for study of Haemoproteus.

KEYWORDS:

Otus lettia; Tyto alba; avian malaria; cytochrome b; raptor

PMID:
29032656
PMCID:
PMC5879076
DOI:
10.4142/jvs.2018.19.2.280
[Indexed for MEDLINE]
Free PMC Article

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