Format

Send to

Choose Destination
EMBO J. 2017 Nov 2;36(21):3194-3211. doi: 10.15252/embj.201797259. Epub 2017 Oct 13.

Aubergine and piRNAs promote germline stem cell self-renewal by repressing the proto-oncogene Cbl.

Author information

1
mRNA Regulation and Development, Institute of Human Genetics, UMR9002 CNRS-Université de Montpellier, Montpellier Cedex 5, France.
2
mRNA Regulation and Development, Institute of Human Genetics, UMR9002 CNRS-Université de Montpellier, Montpellier Cedex 5, France martine.simonelig@igh.cnrs.fr.

Abstract

PIWI proteins play essential roles in germ cells and stem cell lineages. In Drosophila, Piwi is required in somatic niche cells and germline stem cells (GSCs) to support GSC self-renewal and differentiation. Whether and how other PIWI proteins are involved in GSC biology remains unknown. Here, we show that Aubergine (Aub), another PIWI protein, is intrinsically required in GSCs for their self-renewal and differentiation. Aub needs to be loaded with piRNAs to control GSC self-renewal and acts through direct mRNA regulation. We identify the Cbl proto-oncogene, a regulator of mammalian hematopoietic stem cells, as a novel GSC differentiation factor. Aub stimulates GSC self-renewal by repressing Cbl mRNA translation and does so in part through recruitment of the CCR4-NOT complex. This study reveals the role of piRNAs and PIWI proteins in controlling stem cell homeostasis via translational repression and highlights piRNAs as major post-transcriptional regulators in key developmental decisions.

KEYWORDS:

CCR4‐NOT deadenylation complex; PIWI proteins; germline stem cells; piRNAs; translational control

PMID:
29030484
PMCID:
PMC5666619
DOI:
10.15252/embj.201797259
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center