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Plant Physiol. 2017 Dec;175(4):1669-1689. doi: 10.1104/pp.17.01096. Epub 2017 Oct 13.

Genome-Wide Identification of Medicago Peptides Involved in Macronutrient Responses and Nodulation.

Author information

1
Noble Research Institute, Ardmore, Oklahoma 73401.
2
Department of Plant and Environmental Sciences and Copenhagen Plant Science Center, Faculty of Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
3
Noble Research Institute, Ardmore, Oklahoma 73401 pklundquist@noble.org wrscheible@noble.org.
4
Department of Biological Sciences, BioDiscovery Institute, University of North Texas, Denton, Texas 76203.

Abstract

Growing evidence indicates that small, secreted peptides (SSPs) play critical roles in legume growth and development, yet the annotation of SSP-coding genes is far from complete. Systematic reannotation of the Medicago truncatula genome identified 1,970 homologs of established SSP gene families and an additional 2,455 genes that are potentially novel SSPs, previously unreported in the literature. The expression patterns of known and putative SSP genes based on 144 RNA sequencing data sets covering various stages of macronutrient deficiencies and symbiotic interactions with rhizobia and mycorrhiza were investigated. Focusing on those known or suspected to act via receptor-mediated signaling, 240 nutrient-responsive and 365 nodulation-responsive Signaling-SSPs were identified, greatly expanding the number of SSP gene families potentially involved in acclimation to nutrient deficiencies and nodulation. Synthetic peptide applications were shown to alter root growth and nodulation phenotypes, revealing additional regulators of legume nutrient acquisition. Our results constitute a powerful resource enabling further investigations of specific SSP functions via peptide treatment and reverse genetics.

PMID:
29030416
PMCID:
PMC5717731
DOI:
10.1104/pp.17.01096
[Indexed for MEDLINE]
Free PMC Article

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