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Eur J Mass Spectrom (Chichester). 2017 Aug;23(4):209-212. doi: 10.1177/1469066717720907.

Evaluation of plasma peptides extraction methods by high-resolution mass spectrometry.

Author information

1 Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
2 Moscow Institute of Physics and Technology, Dolgoprudny, Russia.
3 School of chemistry, Department of analytical chemistry, Lomonosov Moscow State University, Moscow, Russia.
4 V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
5 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
6 Skolkovo Institute of Science and Technology, Moscow, Russia.


Monitoring of peptides offers a promising approach for the discovery of novel biomarkers, which might be valuable for detection, treatment and prevention of large variety of diseases. Development of highly effective methods for plasma peptide extraction remains an important task. In the current study, we applied different types of plasma peptide extraction approaches to reveal efficient methods which would provide the highest peptide yield. We used different combinations of plasma treatment with acetonitrile and/or urea/guanidine, protein precipitation by acetone, gel-filtration, ultrafiltration, and two types of solid phase extraction. The extracted peptides were analyzed by LC-MS/MS. The obtained results suggest that several methods, including differential solubilization, organic precipitation, as well as some variants of ultrafiltration and solid phase extraction, provide effective plasma peptide enrichment convenient for further LC-MS/MS analysis. Alas, most of the identified peptides were extracted by only one of the applied methods. Hence, it seems reasonable to consider several methods to increase the possibility of novel biomarker discovery.


Plasma peptidome; differential solubilization; high-resolution mass spectrometry; organic precipitation; solid phase extraction; ultrafiltration

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