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Microbes Infect. 2018 Feb;20(2):101-110. doi: 10.1016/j.micinf.2017.10.001. Epub 2017 Oct 10.

Comparative analysis of clinics, pathologies and immune responses in BALB/c and C57BL/6 mice infected with Streptobacillus moniliformis.

Author information

1
Institute for Bacteriology and Mycology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany.
2
Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany.
3
Institute for Veterinary Pathology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany.
4
GVG Diagnostics GmbH, Center for Biotechnology and Biomedicine, Leipzig, Germany; Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University Leipzig, Leipzig, Germany; Centre for Biotechnology and Biomedicine, Leipzig, Germany.
5
Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University Leipzig, Leipzig, Germany; Centre for Biotechnology and Biomedicine, Leipzig, Germany.
6
Hessian State Laboratory Giessen, Giessen, Germany; Institute of Hygiene and Infectious Diseases of Animals, Justus-Liebig-University Giessen, Giessen, Germany.
7
Institute for Immunology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany.
8
Institute for Bacteriology and Mycology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany. Electronic address: christoph.baums@vetmed.uni-leipzig.de.

Abstract

Streptobacillus (S.) moniliformis is a rat-associated zoonotic pathogen that occasionally causes disease in other species. We investigated the working hypothesis that intranasal infection might lead to different immune responses in C57BL/6 and BALB/c mice associated with distinct pathologies. This study confirmed with 75% mortality the known high susceptibility of C57BL/6 mice to Streptobacillus moniliformis infection in comparison to BALB/c mice which did not develop signs of disease. Main pathologies in C57BL/6 mice were purulent to necrotizing lymphadenitis and pneumonia. Significant seroconversion was recorded in surviving mice of both strains. Differentiation of IgG-subclasses revealed mean ratios of IgG2b to IgG1 below 0.5 in sera of all mice prior to infection and of BALB/c mice post infection. In contrast, C57BL/6 mice had a mean IgG2b/IgG1 ratio of 2.5 post infection indicating a Th1 immune response in C57BL/6 versus a Th2 response in BALB/c mice. Evaluation of different sentinel systems revealed that cultural and serological investigations of these animals might not be sufficient to detect infection. In summary, an intranasal S. moniliformis infection model in C57BL/6 mice leading to purulent to necrotizing inflammations in the lung, the lymph nodes and other organs associated with a Th1 immune response is described.

KEYWORDS:

Bronchopneumonia; Lymphadenitis; Sentinel; Th1-associated inflammation

PMID:
29024796
DOI:
10.1016/j.micinf.2017.10.001
[Indexed for MEDLINE]

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