(A-C) Calcium signal from AgRP neurons in fasted mice in response to vehicle (black) or devazepide (dev, A), ondansetron (ods, B), or JNJ-31020028 (jnj, C) (red).
(D) Quantification of ΔF/F from (A-C). Time shown is a 5-min time window 25 min after antagonist administration.
(E and F) Calcium signal in fasted mice after intragastric injection of dev or vehicle followed by intragastric infusion of lipid (E) or glucose (F).
(G and H) Quantification of of ΔF/F from (E and F). n = 4 mice per group.
(I and J) Calcium signal from AgRP neurons in fasted mice after intragastric injection of ods or vehicle followed by intragastric infusion of lipid (I) or glucose (J).
(K and L) Quantification of of ΔF/F from (I and J). n = 4 mice per group.
(M and N) Calcium signal from AgRP neurons in fasted mice after subcutaneous injection of jnj or vehicle followed by intragastric infusion of lipid (M) or glucose (N).
(O and P) Quantification of of ΔF/F from (M and N). n = 4 mice per group.
(A-C, E, F, I, J, M, N) traces represent mean ± SEM
(G, H, K, L, O, P) Times shown are 5-min windows from the early part of infusion (5 min), the end of infusion (20 min), and 10 min following the end of infusion (30 min).
■ denotes individual mice. Bars represent mean ± SEM. *P < 0.05, ***P < 10-3 compared to vehicle at the indicated time point.