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Anat Sci Educ. 2017 Oct 10. doi: 10.1002/ase.1743. [Epub ahead of print]

The histopathologic reliability of tissue taken from cadavers within the gross anatomy laboratory.

Author information

1
Department of Cell Biology and Anatomy School of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana.
2
Department of Pathology School of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana.
3
Leonard J. Chabert Medical Center Ochsner, Health System, Houma, Louisiana.
4
School of Foundations, Leadership, and Administration, Kent State University, Kent, Ohio.

Abstract

The purpose of this study was to examine the histopathologic reliability of embalmed cadaveric tissue taken from the gross anatomy laboratory. Tissue samples from hearts, livers, lungs, and kidneys were collected after the medical students' dissection course was completed. All of the cadavers were embalmed in a formalin-based fixative solution. The tissue was processed, embedded in paraffin, sectioned at six micrometers, and stained with H&E. The microscope slides were evaluated by a board certified pathologist to determine whether the cellular components of the tissues were preserved at a high enough quality to allow for histopathologic diagnosis. There was a statistically significant relationship between ratings and organ groups. Across all organs, there was a smaller proportion of "poor" ratings. The lung group had the highest percentage of "poor" ratings (23.1%). The heart group had the least "poor" ratings (0.0%). The largest percentage of "satisfactory" ratings were in the lung group (52.8%), and the heart group contained the highest percentage of "good" ratings (58.5%) The lung group had the lowest percentage of "good" ratings (24.2%). These results indicate that heart tissue is more reliable than lung, kidney, or liver tissue when utilizing tissue from the gross anatomy laboratory for research and/or educational purposes. This information advises educators and researchers about the quality and histopathologic reliability of tissue samples obtained from the gross anatomy laboratory. Anat Sci Educ. © 2017 American Association of Anatomists.

KEYWORDS:

cadaver biopsy; gross anatomy education; histology education; histopathology; medical education; pathology education

PMID:
29024453
DOI:
10.1002/ase.1743
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