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Elife. 2017 Oct 12;6. pii: e31307. doi: 10.7554/eLife.31307.

Local processing in neurites of VGluT3-expressing amacrine cells differentially organizes visual information.

Author information

1
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, Saint Louis, United States.
2
Graduate Program in Neuroscience, Washington University School of Medicine, Saint Louis, United States.
3
Allen Institute for Brain Science, Seattle, United States.
4
Department of Neuroscience, Washington University School of Medicine, Saint Louis, United States.
5
Department of Biomedical Engineering, Washington University School of Medicine, Saint Louis, United States.
6
Hope Center for Neurological Disorders, Washington University School of Medicine, Saint Louis, United States.

Abstract

Neurons receive synaptic inputs on extensive neurite arbors. How information is organized across arbors and how local processing in neurites contributes to circuit function is mostly unknown. Here, we used two-photon Ca2+ imaging to study visual processing in VGluT3-expressing amacrine cells (VG3-ACs) in the mouse retina. Contrast preferences (ON vs. OFF) varied across VG3-AC arbors depending on the laminar position of neurites, with ON responses preferring larger stimuli than OFF responses. Although arbors of neighboring cells overlap extensively, imaging population activity revealed continuous topographic maps of visual space in the VG3-AC plexus. All VG3-AC neurites responded strongly to object motion, but remained silent during global image motion. Thus, VG3-AC arbors limit vertical and lateral integration of contrast and location information, respectively. We propose that this local processing enables the dense VG3-AC plexus to contribute precise object motion signals to diverse targets without distorting target-specific contrast preferences and spatial receptive fields.

KEYWORDS:

amacrine cell; local processing; mouse; neuroscience; object motion sensitivity; population activity; retina

PMID:
29022876
PMCID:
PMC5653236
DOI:
10.7554/eLife.31307
[Indexed for MEDLINE]
Free PMC Article

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