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Genetics. 2017 Dec;207(4):1423-1440. doi: 10.1534/genetics.117.300383. Epub 2017 Oct 11.

Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.

Author information

1
Department of Biology, Brandeis University, Waltham, Massachusetts 02454.
2
National Center for Behavioral Genomics, Brandeis University, Waltham, Massachusetts 02454.
3
School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland.
4
School of Biochemistry and Immunology, Microscopy Facility, Trinity Biomedical Sciences Institute, Trinity College, Dublin 2, Ireland.
5
Department of Biology, Brandeis University, Waltham, Massachusetts 02454 sengupta@brandeis.edu oliver.blacque@ucd.ie.
6
School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland sengupta@brandeis.edu oliver.blacque@ucd.ie.

Abstract

Motile and immotile (or primary) cilia are microtubule-based structures that mediate multiple cellular functions, including the transduction of environmental cues, developmental signaling, cellular motility, and modulation of fluid flow. Although their core architectures are similar, motile and primary cilia exhibit marked structural differences that underlie distinct functional properties. However, the extent to which ciliogenesis mechanisms are shared between these different cilia types is not fully described. Here, we report that the atypical MAP kinase MAPK15 (ERK7/8), implicated in the formation of vertebrate motile cilia, also regulates the formation of primary cilia in Caenorhabditis elegans sensory neurons and human cells. We find that MAPK15 localizes to a basal body subdomain with the ciliopathy protein BBS7 and to cell-cell junctions. MAPK15 also regulates the localization of ciliary proteins involved in cilium structure, transport, and signaling. Our results describe a primary cilia-related role for this poorly studied member of the MAPK family in vivo, and indicate a broad requirement for MAPK15 in the formation of multiple ciliary classes across species.

KEYWORDS:

C. elegans; MAPK15; basal body; primary cilia

PMID:
29021280
PMCID:
PMC5714457
DOI:
10.1534/genetics.117.300383
[Indexed for MEDLINE]
Free PMC Article

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