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Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. Epub 2017 Oct 9.

HIV-1 subtype CRF01_AE and B differ in utilization of low levels of CCR5, Maraviroc susceptibility and potential N-glycosylation sites.

Author information

1
Department of Biomedical Sciences, Texas Tech University Health Sciences Center, El Paso, TX, USA. Electronic address: anjali.joshi@ttuhsc.edu.
2
Department of Biomedical Sciences, Texas Tech University Health Sciences Center, El Paso, TX, USA.
3
Bioinformatics Institute, Agency for Science, Technology and Research, Singapore.
4
Bioinformatics Institute, Agency for Science, Technology and Research, Singapore; Department of Biological Sciences, National University of Singapore, Singapore.
5
Department of Infectious Disease, Tan Tock Seng Hospital, Singapore.
6
Department of Biomedical Sciences, Texas Tech University Health Sciences Center, El Paso, TX, USA. Electronic address: himanshu.garg@ttuhsc.edu.

Abstract

HIV subtypes not only predominate in different geographical regions but also differ in key phenotypic characteristics. To determine if genotypic and/or phenotypic differences in the Envelope (Env) glycoprotein can explain subtype related differences, we cloned 37 full length Envs from Subtype B and AE HIV infected individuals from Singapore. Our data demonstrates that CRF01_AE Envs have lower Potential N Glycosylation Sites and higher risk of ×4 development. Phenotypically, CRF01_AE were less infectious than subtype B Envs in cells expressing low levels of CCR5. Moreover, the Maraviroc IC50 was higher for subtype B Envs and correlated with infectivity in low CCR5 expressing cells as well as PNGS. Specifically, the glycosylation site N301 in the V3 loop was seen less frequently in AE subtype and CXCR4 topic viruses. CRF01_AE differs from B subtype in terms of CCR5 usage and Maraviroc susceptibility which may have implications for HIV pathogenesis and virus evolution.

KEYWORDS:

AIDS; CCR5; CD4; CRF01_AE; Envelope; HIV; Maraviroc; PNGS; Subtype B; gp120

PMID:
29020646
PMCID:
PMC5653400
[Available on 2018-12-01]
DOI:
10.1016/j.virol.2017.09.026
[Indexed for MEDLINE]
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