Format

Send to

Choose Destination
J Am Heart Assoc. 2017 Oct 10;6(10). pii: e006837. doi: 10.1161/JAHA.117.006837.

Peptidyl-Prolyl Isomerase 1 Regulates Ca2+ Handling by Modulating Sarco(Endo)Plasmic Reticulum Calcium ATPase and Na2+/Ca2+ Exchanger 1 Protein Levels and Function.

Author information

1
The San Diego Heart Research Institute and the Department of Biology, San Diego State University, San Diego, CA.
2
Department of Pharmacology, UC Davis, Davis, CA.
3
Molecular Enzymology, Department of Molecular Cell Science, Graduate School of Agricultural Science, Tohoku University, Miyagi, Japan.
4
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX.
5
Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA.
6
Department of Physiology, Basic Science Building New York Medical College, Valhalla, NY.
7
The San Diego Heart Research Institute and the Department of Biology, San Diego State University, San Diego, CA heartman4ever@icloud.com.

Abstract

BACKGROUND:

Aberrant Ca2+ handling is a prominent feature of heart failure. Elucidation of the molecular mechanisms responsible for aberrant Ca2+ handling is essential for the development of strategies to blunt pathological changes in calcium dynamics. The peptidyl-prolyl cis-trans isomerase peptidyl-prolyl isomerase 1 (Pin1) is a critical mediator of myocardial hypertrophy development and cardiac progenitor cell cycle. However, the influence of Pin1 on calcium cycling regulation has not been explored. On the basis of these findings, the aim of this study is to define Pin1 as a novel modulator of Ca2+ handling, with implications for improving myocardial contractility and potential for ameliorating development of heart failure.

METHODS AND RESULTS:

Pin1 gene deletion or pharmacological inhibition delays cytosolic Ca2+ decay in isolated cardiomyocytes. Paradoxically, reduced Pin1 activity correlates with increased sarco(endo)plasmic reticulum calcium ATPase (SERCA2a) and Na2+/Ca2+ exchanger 1 protein levels. However, SERCA2a ATPase activity and calcium reuptake were reduced in sarcoplasmic reticulum membranes isolated from Pin1-deficient hearts, suggesting that Pin1 influences SERCA2a function. SERCA2a and Na2+/Ca2+ exchanger 1 associated with Pin1, as revealed by proximity ligation assay in myocardial tissue sections, indicating that regulation of Ca2+ handling within cardiomyocytes is likely influenced through Pin1 interaction with SERCA2a and Na2+/Ca2+ exchanger 1 proteins.

CONCLUSIONS:

Pin1 serves as a modulator of SERCA2a and Na2+/Ca2+ exchanger 1 Ca2+ handling proteins, with loss of function resulting in impaired cardiomyocyte relaxation, setting the stage for subsequent investigations to assess Pin1 dysregulation and modulation in the progression of heart failure.

KEYWORDS:

Na+/Ca2+ exchange; cardiomyocyte; peptidyl‐prolyl isomerase 1; sarcoplasmic reticulum Ca2+‐ATPase

PMID:
29018025
PMCID:
PMC5721875
DOI:
10.1161/JAHA.117.006837
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center