Format

Send to

Choose Destination
EMBO J. 1988 Jul;7(7):2191-201.

Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter.

Author information

1
Department of Biology, Washington University, St. Louis, MO 63130.

Erratum in

  • EMBO J 1988 Oct;7(10):3300.

Abstract

Genomic footprinting on the Drosophila hsp26 promoter in isolated nuclei has shown that a TATA box binding factor is present before and after induction by heat shock, while three of the seven heat shock consensus sequences 5' of the gene are occupied (presumably by heat shock factor, HSF) specifically on heat shock. The sites of HSF interaction are separated by greater than 200 bp of which approximately 150 bp are bound to the surface of a nucleosome. The juxtaposition of these various macromolecules on the DNA suggests a basis for the major DNase I hypersensitive site 5' of hsp26 and a novel tertiary structure for the promoter complex.

PMID:
2901349
PMCID:
PMC454544
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center