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J Vis Exp. 2017 Sep 22;(127). doi: 10.3791/56017.

Cardiopulmonary Bypass in a Mouse Model: A Novel Approach.

Author information

1
Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School; Madrahimov.Nodir@mh-hannover.de.
2
Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School.
3
Department of Hematology, Oncology, Immunology, Rheumatology, and Pulmonology, University Hospital Tuebingen.
4
Department of Pneumology, Hannover Medical School.

Abstract

As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization and for minimizing organ damage resulting from prolonged extracorporal circulation. The goal of this paper was to demonstrate a fully functional and clinically relevant model of cardiopulmonary bypass in a mouse. We report on the device design, perfusion circuit optimization, and microsurgical techniques. This model is an acute model, which is not compatible with survival due to the need for multiple blood drawings. Because of the range of tools available for mice (e.g., markers, knockouts, etc.), this model will facilitate investigation into the molecular mechanisms of organ damage and the effect of cardiopulmonary bypass in relation to other comorbidities.

PMID:
28994765
PMCID:
PMC5752326
DOI:
10.3791/56017
[Indexed for MEDLINE]
Free PMC Article

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