Format

Send to

Choose Destination
J Exp Bot. 2017 Oct 13;68(17):4821-4837. doi: 10.1093/jxb/erx284.

The plasma membrane H+-ATPase gene family in Solanum tuberosum L. Role of PHA1 in tuberization.

Author information

1
Institute of Genetic Engineering and Molecular Biology 'Dr. Héctor Torres' (INGEBI), National Research Council (CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina.
2
Biochemistry Department, School of Exact and Natural Sciences, University of Buenos Aires, Buenos Aires, Argentina.

Abstract

This study presents the characterization of the plasma membrane (PM) H+-ATPases in potato, focusing on their role in stolon and tuber development. Seven PM H+-ATPase genes were identified in the Solanum tuberosum genome, designated PHA1-PHA7. PHA genes show distinct expression patterns in different plant tissues and under different stress treatments. Application of PM H+-ATPase inhibitors arrests stolon growth, promotes tuber induction, and reduces tuber size, indicating that PM H+-ATPases are involved in tuberization, acting at different stages of the process. Transgenic potato plants overexpressing PHA1 were generated (PHA1-OE). At early developmental stages, PHA1-OE stolons elongate faster and show longer epidermal cells than wild-type stolons; this accelerated growth is accompanied by higher cell wall invertase activity, lower starch content, and higher expression of the sucrose-H+ symporter gene StSUT1. PHA1-OE stolons display an increased branching phenotype and develop larger tubers. PHA1-OE plants are taller and also present a highly branched phenotype. These results reveal a prominent role for PHA1 in plant growth and development. Regarding tuberization, PHA1 promotes stolon elongation at early stages, and tuber growth later on. PHA1 is involved in the sucrose-starch metabolism in stolons, possibly providing the driving force for sugar transporters to maintain the apoplastic sucrose transport during elongation.

KEYWORDS:

Branching; PHA1; PM H+-ATPase; plant growth; potato; stolon elongation; tuber growth; tuberization

PMID:
28992210
PMCID:
PMC5853856
DOI:
10.1093/jxb/erx284
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center