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Nat Methods. 2017 Oct 9. doi: 10.1038/nmeth.4461. [Epub ahead of print]

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV.

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1
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA.

Abstract

Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.

PMID:
28991891
DOI:
10.1038/nmeth.4461
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