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Nat Genet. 2017 Nov;49(11):1633-1641. doi: 10.1038/ng.3972. Epub 2017 Oct 9.

The draft genome of tropical fruit durian (Durio zibethinus).

Teh BT1,2,3,4,5,6, Lim K2,7, Yong CH2,7, Ng CCY3, Rao SR8,9,10,11, Rajasegaran V3, Lim WK2,4,7, Ong CK10, Chan K12, Cheng VKY13, Soh PS14, Swarup S8,9,10,11,15, Rozen SG2,4,7, Nagarajan N16, Tan P1,2,4,5,16.

Author information

1
Thorn Biosystems Pte Ltd, Singapore.
2
Program in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore.
3
Laboratory of Cancer Epigenome, Division of Medical Science, National Cancer Centre singapore, Singapore.
4
SingHealth/Duke-NUS Institute of Precision Medicine, National Heart Centre, Singapore.
5
Cancer Science Institute of Singapore, National University of Singapore, Singapore.
6
Institute of Molecular and Cellular Biology, Singapore.
7
Centre for Computational Biology, Duke-NUS Medical School, Singapore.
8
Department of Biological Sciences, National University of Singapore, Singapore.
9
Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore.
10
Metabolites Biology Laboratory, National University of Singapore, Singapore.
11
NUS Synthetic Biology for Clinical and Technological Innovation, Life Sciences Institute, National University of Singapore, Singapore.
12
Global Databank, Singapore.
13
Verdant Foundation, Hong Kong.
14
Samsoney Group, Johor Bahru, Malaysia.
15
NUS Environmental Research Institute, National University of Singapore, Singapore.
16
Genome Institute of Singapore, Singapore.

Abstract

Durian (Durio zibethinus) is a Southeast Asian tropical plant known for its hefty, spine-covered fruit and sulfury and onion-like odor. Here we present a draft genome assembly of D. zibethinus, representing the third plant genus in the Malvales order and first in the Helicteroideae subfamily to be sequenced. Single-molecule sequencing and chromosome contact maps enabled assembly of the highly heterozygous durian genome at chromosome-scale resolution. Transcriptomic analysis showed upregulation of sulfur-, ethylene-, and lipid-related pathways in durian fruits. We observed paleopolyploidization events shared by durian and cotton and durian-specific gene expansions in MGL (methionine γ-lyase), associated with production of volatile sulfur compounds (VSCs). MGL and the ethylene-related gene ACS (aminocyclopropane-1-carboxylic acid synthase) were upregulated in fruits concomitantly with their downstream metabolites (VSCs and ethylene), suggesting a potential association between ethylene biosynthesis and methionine regeneration via the Yang cycle. The durian genome provides a resource for tropical fruit biology and agronomy.

PMID:
28991254
DOI:
10.1038/ng.3972
[Indexed for MEDLINE]

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