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Elife. 2017 Oct 9;6. pii: e28935. doi: 10.7554/eLife.28935.

Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity.

Author information

1
Institut des Neurosciences Cellulaires et Intégratives, CNRS, Université de Strasbourg, Strasbourg, France.
2
Carl-Ludwig Institute for Physiology, University of Leipzig, Leipzig, Germany.

Abstract

The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We show that at low-frequency stimulations, a first sub-pool is gradually silenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synaptic vesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRP in terminals of granule cells may play a role in the filtering of sensorimotor information in the cerebellum.

KEYWORDS:

Purkinje cell; cerebellum; mouse; neuroscience; neurotransmitter release; parallel fibers; short-term plasticity; synaptic vesicles

PMID:
28990927
PMCID:
PMC5648531
DOI:
10.7554/eLife.28935
[Indexed for MEDLINE]
Free PMC Article

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