Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2017 Dec 2;493(4):1498-1503. doi: 10.1016/j.bbrc.2017.10.015. Epub 2017 Oct 4.

DCPP1 is the mouse ortholog of human PAUF that possesses functional analogy in pancreatic cancer.

Author information

1
Department of Biological Sciences, Dong-A University, Busan, South Korea.
2
Aging Research Institute, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.
3
Department of Biological Sciences, Dong-A University, Busan, South Korea. Electronic address: sskoh@dau.ac.kr.

Abstract

Pancreatic adenocarcinoma upregulated factor (PAUF) overexpressed in pancreatic ductal adenocarcinoma (PDAC) plays a major role in tumor progression and metastasis by autocrine and paracrine manners. However, underlying molecular mechanism of PAUF functioning in pancreatic cancer are not fully understood yet. The objective of this study was to evaluate the potential of demilune cell and parotid protein 1 (DCPP1) as a putative mouse ortholog of human PAUF by sequence alignment and functional studies. Overexpression of mouse DCPP1 in Chinese hamster ovary (CHO) cells or pancreatic cancer cells increased cell proliferation, migration, invasion, and adhesion ability in vitro. Treatment of human pancreatic cancer cells with recombinant mouse DCPP1 elevated cell growth, motility, invasiveness, and adhesiveness. Mouse DCPP1 exerted its function on pancreatic cancer cells by activating intracellular signaling pathways involved in aggressive cancer phenotype of human pancreatic cancer cells. Moreover, subcutaneous injection of mice with DCPP1-overexpressing CHO cells increased tumor sizes. Taken together, we conclude that mouse DCPP1 is a multifunctional promoter of tumor growth through functional activation of pancreatic cancer cells, suggesting it to be an ortholog of human PAUF.

KEYWORDS:

Cancer progression; DCPP1; Ortholog; PAUF; Pancreatic cancer

PMID:
28988106
DOI:
10.1016/j.bbrc.2017.10.015
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center