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Eur Radiol. 2018 Mar;28(3):1149-1156. doi: 10.1007/s00330-017-5056-9. Epub 2017 Oct 6.

Absence of DNA double-strand breaks in human peripheral blood mononuclear cells after 3 Tesla magnetic resonance imaging assessed by γH2AX flow cytometry.

Author information

1
Department of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Göttingen Heart Center, Robert-Koch-Str. 40, 37075, Göttingen, Germany. martin.fasshauer@med.uni-goettingen.de.
2
German Centre for Cardiovascular Research (DZHK), Göttingen, Germany. martin.fasshauer@med.uni-goettingen.de.
3
Department of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Göttingen Heart Center, Robert-Koch-Str. 40, 37075, Göttingen, Germany.
4
Department of Medical Statistics, University Medical Center Göttingen, Göttingen, Germany.
5
Department of Radiotherapy and Radiooncology, University Medical Center Göttingen, Göttingen, Germany.
6
German Centre for Cardiovascular Research (DZHK), Göttingen, Germany.
7
Department of Pediatric Cardiology and Intensive Care Medicine, University Medical Center Göttingen, Göttingen Heart Center, Göttingen, Germany.
8
Department of Cardiology and Pneumology, University Medical Center Göttingen, Göttingen Heart Center, Göttingen, Germany.

Abstract

OBJECTIVES:

Magnetic resonance imaging (MRI) is regarded as a non-harming and non-invasive imaging modality with high tissue contrast and almost no side effects. Compared to other cross-sectional imaging modalities, MRI does not use ionising radiation. Recently, however, strong magnetic fields as applied in clinical MRI scanners have been suspected to induce DNA double-strand breaks in human lymphocytes.

METHODS:

In this study we investigated the impact of 3-T cardiac MRI examinations on the induction of DNA double-strand breaks in peripheral mononuclear cells by γH2AX staining and flow cytometry analysis. The study cohort consisted of 73 healthy non-smoking volunteers with 36 volunteers undergoing CMRI and 37 controls without intervention. Differences between the two cohorts were analysed by a mixed linear model with repeated measures.

RESULTS:

Both cohorts showed a significant increase in the γH2AX signal from baseline to post-procedure of 6.7 % (SD 7.18 %) and 7.8 % (SD 6.61 %), respectively. However, the difference between the two groups was not significant.

CONCLUSION:

Based on our study, γH2AX flow cytometry shows no evidence that 3-T MRI examinations as used in cardiac scans impair DNA integrity in peripheral mononuclear cells.

KEY POINTS:

• No evidence for DNA double-strand breaks after cardiac MRI. • Prospective study underlines safe use of MRI with regard to DNA damage. • Controlled trial involving both genders investigating DNA DSBs after 3-T MRI.

KEYWORDS:

Cardiac imaging; Double-stranded DNA breaks; FACS; Gamma-H2AX foci assay; MRI safety

PMID:
28986695
DOI:
10.1007/s00330-017-5056-9
[Indexed for MEDLINE]

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