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Int Immunopharmacol. 2017 Nov;52:318-323. doi: 10.1016/j.intimp.2017.09.028. Epub 2017 Oct 3.

Circulating CD4+CXCR5+ T cells contribute to proinflammatory responses in multiple ways in coronary artery disease.

Author information

1
Department of Cardiology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
2
Department of Orthopedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China.
3
Medical Department, The Maternal and Child Health Hospital of Jinan City, Jinan, Shandong 250001, China.
4
Department of Cardiology, East Hospital, Tongji University School of Medicine, Shanghai 200120, China. Electronic address: malanseh@hotmail.com.
5
Department of Cardiology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. Electronic address: chunliangcz@126.com.

Abstract

Coronary artery disease (CAD) is a common subtype of cardiovascular disease. The major contributing event is atherosclerosis, which is a progressive inflammatory condition resulting in the thickening of the arterial wall and the formation of atheromatous plaques. Recent evidence suggests that circulating CD4+CXCR5+ T cells can contribute to inflammatory reactions. In this study, the frequency, phenotype, and function of circulating CD4+CXCR5+ T cells in CAD patients were examined. Data showed that circulating CD4+CXCR5+ T cells in CAD patients were enriched with a PD-1+CCR7- subset, which was previously identified as the most potent in B cell help. The CD4+CXCR5+ T cells in CAD patients also secreted significantly higher levels of IFN-γ, IL-17A, and IL-21 than those from healthy controls. Depleting the PD-1+ population significantly reduced the cytokine secretion. Interestingly, the CD4+CXCR5+PD-1- T cells significantly upregulated PD-1 following anti-CD3/CD28 or SEB stimulation. CD4+CXCR5+ T cells from CAD patients also demonstrated more potent capacity to stimulate B cell inflammation than those from healthy individuals. The phosphorylation of STAT1 and STAT3 were significantly higher in B cells incubated with CD4+CXCR5+ T cells from CAD than controls. The IL-6 and IFN-γ expression were also significantly higher in B cells incubated with CD4+CXCR5+ T cells from CAD. Together, this study demonstrated that CAD patients presented a highly activated CD4+CXCR5+ T cell subset that could contribute to proinflammatory responses in multiple ways. The possibility of using CD4+CXCR5+ T cells as a therapeutic target should therefore be examined in CAD patients.

KEYWORDS:

CD4(+)CXCR5(+) T cell; Coronary artery disease; Tfh

PMID:
28985621
DOI:
10.1016/j.intimp.2017.09.028
[Indexed for MEDLINE]

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