Format

Send to

Choose Destination
Development. 2017 Nov 1;144(21):4037-4045. doi: 10.1242/dev.155176. Epub 2017 Oct 5.

The Dlx5-FGF10 signaling cascade controls cranial neural crest and myoblast interaction during oropharyngeal patterning and development.

Author information

1
Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA 90033, USA.
2
Division of Endodontology, Kyushu University Hospital, Kyushu University, Fukuoka 812-8582, Japan.
3
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
4
Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatological, Beijing 100081, China.
5
Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
6
Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA 90033, USA ychai@usc.edu.

Abstract

Craniofacial development depends on cell-cell interactions, coordinated cellular movement and differentiation under the control of regulatory gene networks, which include the distal-less (Dlx) gene family. However, the functional significance of Dlx5 in patterning the oropharyngeal region has remained unknown. Here, we show that loss of Dlx5 leads to a shortened soft palate and an absence of the levator veli palatini, palatopharyngeus and palatoglossus muscles that are derived from the 4th pharyngeal arch (PA); however, the tensor veli palatini, derived from the 1st PA, is unaffected. Dlx5-positive cranial neural crest (CNC) cells are in direct contact with myoblasts derived from the pharyngeal mesoderm, and Dlx5 disruption leads to altered proliferation and apoptosis of CNC and muscle progenitor cells. Moreover, the FGF10 pathway is downregulated in Dlx5-/- mice, and activation of FGF10 signaling rescues CNC cell proliferation and myogenic differentiation in these mutant mice. Collectively, our results indicate that Dlx5 plays crucial roles in the patterning of the oropharyngeal region and development of muscles derived from the 4th PA mesoderm in the soft palate, likely via interactions between CNC-derived and myogenic progenitor cells.

KEYWORDS:

4th pharyngeal arch; CNC cells; Dlx5; Myoblast; Oropharyngeal patterning; Soft palate

PMID:
28982687
PMCID:
PMC5702075
DOI:
10.1242/dev.155176
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center