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Toxicol In Vitro. 2018 Feb;46:86-93. doi: 10.1016/j.tiv.2017.09.030. Epub 2017 Oct 2.

Endocrine disruptor effect of perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) on porcine ovarian cell steroidogenesis.

Author information

1
Laboratorio de Biología Celular, Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana, Unidad Iztapalapa, CDMX, México; Doctorado en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa, CDMX, México. Electronic address: andych022003@yahoo.com.mx.
2
Laboratorio de Biología Celular, Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana, Unidad Iztapalapa, CDMX, México. Electronic address: bet@xanum.uam.mx.
3
Unidad de Investigación en Biología de la Reproducción, Laboratorio de Neuroinmuno-endocrinología, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, CDMX, México. Electronic address: prosas44@yahoo.com.mx.
4
Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Juriquilla, Querétaro, México. Electronic address: fvazquezc132005@yahoo.com.mx.
5
Departamento de Biología de la Reproducción, Instituto Nacional de la Nutrición "Salvador Zubirán", CDMX, México. Electronic address: robertochavira2002@yahoo.com.mx.
6
Laboratorio de Biología Celular, Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana, Unidad Iztapalapa, CDMX, México. Electronic address: mundo@xanum.uam.mx.
7
Laboratorio de Biología Celular, Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana, Unidad Iztapalapa, CDMX, México. Electronic address: dino@xanum.uam.mx.
8
Laboratorio de Biología Celular, Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana, Unidad Iztapalapa, CDMX, México. Electronic address: duco@xanum.uam.mx.

Abstract

Previous studies with perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) indicate that they act as endocrine disruptors, in addition to inducing alterations and damaging reproductive health; however, the biological mechanisms by which these disorders are produced are not yet understood. The aim of this study was to analyze the effect of PFOS and PFOA on in vitro steroidogenic secretion in porcine theca and granulosa cells, with or without gonadotropic stimulation. Granulosa and theca cells were isolated and cultured. Cell nature was performed by immunocytochemistry. PFOS and PFOA effect on steroid secretion was analyzed by chemiluminescence. In the present study, alterations in steroidogenic secretion were found when administering PFOS (0.12, 1.2, 12, 120 or 240μM) or PFOA (0.012, 0.12, 1.2, 12 or 24μM) to theca and granulosa cells. When theca and granulosa cells were stimulated with 500ng/mL LH or 500ng/mL FHS, respectively and immediately followed with 1.2μM of PFOS or PFOA, the perfluorinated compounds inhibited the secretion of steroid hormones in both stimulated cell types. The results indicate that PFOS and PFOA act on steroidogenic ovarian cells as endocrine disruptors, which could affect the dependent functions of sexual steroids.

KEYWORDS:

Endocrine disruptor; Ovarian cells; PFOA; PFOS; Porcine; Sexual steroids

PMID:
28982594
DOI:
10.1016/j.tiv.2017.09.030
[Indexed for MEDLINE]

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