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Stem Cells Transl Med. 2017 Nov;6(11):1987-1996. doi: 10.1002/sctm.17-0074. Epub 2017 Oct 5.

Long-Term Recovery After Endothelial Colony-Forming Cells or Human Umbilical Cord Blood Cells Administration in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy.

Author information

1
Aix Marseille Univ, INSERM, VRCM, UMR_1076, UFR de Pharmacie, Marseille, France.
2
APHM, CHU La Conception, Department of Neonatology, Marseille, France.
3
APHM, Radiopharmacy, Marseille, France.
4
CERIMED, Aix Marseille Univ, Marseille, France.
5
Division of Pediatrics, CHUV & University of Lausanne, Switzerland.
6
APHM, CHU La Conception, Cell Culture and Therapy Laboratory, INSERM CBT-1409, Marseille, France.

Abstract

Neonatal hypoxic-ischemic encephalopathy (NHIE) is a dramatic perinatal complication, associated with poor neurological prognosis despite neuroprotection by therapeutic hypothermia, in the absence of an available curative therapy. We evaluated and compared ready-to-use human umbilical cord blood cells (HUCBC) and bankable but allogeneic endothelial progenitors (ECFC) as cell therapy candidate for NHIE. We compared benefits of HUCBC and ECFC transplantation 48 hours after injury in male rat NHIE model, based on the Rice-Vannucci approach. Based on behavioral tests, immune-histological assessment and metabolic imaging of brain perfusion using single photon emission computed tomography (SPECT), HUCBC, or ECFC administration provided equally early and sustained functional benefits, up to 8 weeks after injury. These results were associated with total normalization of injured hemisphere cerebral blood flow assessed by SPECT/CT imaging. In conclusion, even if ECFC represent an efficient candidate, HUCBC autologous criteria and easier availability make them the ideal candidate for hypoxic-ischemic cell therapy. Stem Cells Translational Medicine 2017;6:1987-1996.

KEYWORDS:

Endothelial colony-forming cells; Human umbilical cord blood cells; Neonatal hypoxic ischemic encephalopathy; Rat model; Single photon emission computed tomography

PMID:
28980775
DOI:
10.1002/sctm.17-0074
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