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Int J Lab Hematol. 2018 Apr;40(2):136-143. doi: 10.1111/ijlh.12753. Epub 2017 Oct 4.

A ten-color tube with dried antibody reagents for the screening of hematological malignancies.

Author information

1
Clinical Pathology Laboratory, Division of Hematology and Flow Cytometry, Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.
2
Hematology Department, LifeLabs Medical Laboratory Services, Toronto, ON, Canada.
3
Centro de Hematologia de São Paulo, São Paulo, SP, Brazil.

Abstract

INTRODUCTION:

The workflow in clinical flow cytometry laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity and reduce costs. Using the Beckman Coulter dry coating technology, we customized a ten-color tube with dried antibody reagents, designated the Duraclone screening tube (DST), for screening hematological malignancies. Here, we compared the applicability, clinical and numerical equivalence, and cost and time required for the technical procedures between the liquid reagents and the DST.

METHODS:

The DST contains CD4 + Kappa-FITC, CD8 + Lambda-PE, CD3 + CD14-ECD, CD33-PE-Cy5.5, CD20 + CD56-PE-Cy7, CD34-APC, CD19-APC-AlexaFluor700, CD10-APC-AlexaFluor750, CD5-Pacific Blue, and CD45-Krome Orange. We evaluated 20 bone marrow samples, 13 peripheral blood samples, 6 lymph node biopsy samples, 5 fine-needle aspirate samples, 5 cerebrospinal fluid samples, and 1 pleural fluid sample.

RESULTS:

The DST was useful for more than 60% of our samples. It was able to enumerate the majority of the populations in all types of samples with a statistically acceptable correlation with the liquid reagents. The use of the DST translated into significant time and cost savings of 15.8% and 12.3%, respectively, compared with the use of the liquid reagent. The cost was reduced by $14.36 per sample.

CONCLUSIONS:

The DST is an efficient solution for screening hematological malignancies with improved quality, productivity, standardization, and sustainability. These improvements could benefit patients by providing faster diagnoses using a higher quality and lower cost reagent.

KEYWORDS:

flow cytometry; hematological neoplasms; indicators and reagents; staining and labeling

PMID:
28980400
DOI:
10.1111/ijlh.12753
[Indexed for MEDLINE]

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