Format

Send to

Choose Destination
World J Orthop. 2017 Sep 18;8(9):719-725. doi: 10.5312/wjo.v8.i9.719. eCollection 2017 Sep 18.

Association of adiponectin gene polymorphisms with knee osteoarthritis.

Author information

1
Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand.
2
Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand.
3
Vinai Parkpian Orthopaedic Research Center, Department of Orthopaedics, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand.

Abstract

AIM:

To investigate the possible relationship of adiponectin (ADIPOQ) gene polymorphisms, plasma adiponectin, and the risk of knee osteoarthritis (OA).

METHODS:

A total of 398 subjects, 202 knee OA patients and 196 healthy individuals, were enrolled in the case-control study. Genotyping at +45T/G (rs2241766) and +276G/T (rs1501299) loci was performed using polymerase chain reaction-restriction fragment length polymorphism. Plasma adiponectin levels were assessed using enzyme-linked immunosorbent assay. OA severity was determined using the Kellgren-Lawrence (KL) grading system.

RESULTS:

No significant associations were observed in the genotype distributions and allele frequencies at two loci of +45T/G and +276G/T polymorphisms in the ADIPOQ between knee OA patients and control subjects. There was a significant association between genotype distribution of +276G/T polymorphism and KL grade 2, 3 or 4 (P = 0.037, P = 0.046, P = 0.016, respectively). At +45T/G locus, the percentage of GG genotype was notably greater in control subjects (13.40%) compared with OA subjects (1.70%) (P = 0.023). Plasma adiponectin was markedly decreased in OA subjects compared with control subjects (P = 0.03). Likewise, circulating adiponectin in OA subjects was notably lesser than that in control subjects in GG genotype of +45T/G (P = 0.029) and +276G/T polymorphisms (P = 0.012).

CONCLUSION:

Polymorphisms +45T/G and +276G/T of the ADIPOQ gene might not be responsible for OA susceptibility among Thais.

KEYWORDS:

ADIPOQ; Adiponectin; Knee osteoarthritis; Plasma; Polymorphism

Conflict of interest statement

Conflict-of-interest statement: The authors declare that they have no conflict of interest.

Supplemental Content

Full text links

Icon for Baishideng Publishing Group Inc. Icon for PubMed Central
Loading ...
Support Center