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FEBS Open Bio. 2017 Aug 30;7(10):1469-1479. doi: 10.1002/2211-5463.12274. eCollection 2017 Oct.

miR-30d is related to asbestos exposure and inhibits migration and invasion in NCI-H2452 cells.

Author information

1
Institute of Occupational Diseases Zhejiang Academy of Medical Sciences (ZJAMS) Hangzhou Zhejiang China.
2
Jiading District Center for Disease Control and Prevention Shanghai China.
3
Experimental Animal Center Zhejiang Academy of Medical Sciences (ZJAMS) Hangzhou Zhejiang China.

Abstract

Pleural malignant mesothelioma (MM) is a highly aggressive tumor that is typically related to asbestos exposure and has a latency of 20-60 years. Several microRNA contribute to MM initiation and progression, but the mechanisms are not clear. Here, we found that miR-30d is downregulated in the pleural MM cell line NCI-H2452, in the plasma of asbestos-exposed individuals, and in asbestos-exposed mesothelial cells. Furthermore, we investigated the influence of the overexpression of miR-30d in pleural MM cells. We demonstrated that miR-30d overexpression could suppress pleural MM cell proliferation, migration, and invasion in vitro and could promote cell apoptosis but could not significantly influence cell cycle. The mRNA and protein expression of vimentin and TWIST1 decreased, and the mRNA expression of CDH1 increased in NCI-H2452 cells that overexpressed miR-30d. We therefore conclude that miR-30d is related to asbestos exposure and inhibits cell migration and invasion by regulating the epithelial-mesenchymal transition in NCI-H2452 cells.

KEYWORDS:

asbestos; mesothelioma; microRNA; microRNA‐30d; neoplasm invasiveness

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