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Biochem Biophys Res Commun. 1988 May 16;152(3):1361-8.

Clostridium spiroforme toxin is a binary toxin which ADP-ribosylates cellular actin.

Author information

1
Unité des Anaréobies, UA CNRS 557, Paris, France.

Abstract

We have purified from Clostridium spiroforme strain 246 an heterogeneous population of proteins (Sa) ranging from 43 to 47 kilodaltons exhibiting ADP-ribosyl transferase activity as do C. botulinum C2 toxin component I or the ia chain of C. perfringens E iota toxin. C. spiriforme Sa had alone no activity upon injection in mice or inoculated to Vero cells. When spiroforme ADP ribosyl transferase were mixed with a trypsin activated protein (Sb) separated from C. spiroforme bacterial supernatant, a lethal effect in mice and cytotoxicity on Vero cells were recorded. The Sa cross-reacted immunologically with either the light chain of C. perfringens E iota toxin or the ADP-ribosyl transferase from C. difficile 196 strain. No immunological relatedness was observed between Sa and C2 toxin component I. C. spiroforme toxin is thus another binary toxin close to iota.

PMID:
2897847
DOI:
10.1016/s0006-291x(88)80435-2
[Indexed for MEDLINE]

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