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J Asian Nat Prod Res. 2018 Dec;20(12):1182-1193. doi: 10.1080/10286020.2017.1384819. Epub 2017 Oct 5.

Ameliorative effects of gallic acid on gentamicin-induced nephrotoxicity in rats.

Author information

1
a Cellular and Molecular Research Center , Zahedan University of Medical Sciences , Zahedan , Iran.
2
b Department of Physiology and Pharmacology , Rafsanjan University of Medical Sciences , Rafsanjan , Iran.
3
c Physiology-Pharmacology Research Center , Rafsanjan University of Medical Sciences , Rafsanjan , Iran.
4
d Medicinal Plant Research Center , Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran.
5
e Department of Pediatric Nephrology , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
6
f Physiology Research Center, Institute of Neuropharmacology , Kerman University of Medical Sciences , Kerman , Iran.
7
g Department of Pharmacology, School of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
8
h Shoushtar Faculty of Medical Sciences , Shoushtar , Iran.
9
i Razi Drug Research Center, School of Medicine , Iran University of Medical Sciences , Tehran , Iran.
10
j Health Promotion Research Center , Iran University of Medical Sciences , Tehran , Iran.
11
k Student Research Committee , Ahvaz Jundishapur University of Medical Sciences , Ahvaz , Iran.

Abstract

The major side effect of gentamicin (GEN) is nephrotoxicity which in turn restricts the clinical use of this drug. In this study, the effect of gallic acid (GA) on gentamicin-induced nephrotoxicity was studied. A total number of 28 male Wistar rats were randomly divided into four experimental groups: control, GEN (100 mg/kg/day), GEN + GA (30 mg/kg/day), GA (30 mg/kg/day). All drug administrations were done intraperitoneally (i.p) for eight consecutive days. Twenty-four hours after the last administration, blood samples were collected to determine serum creatinine (Cr), blood urea nitrogen (BUN). The right kidney was used for histological examination. Malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO) levels and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity were assayed in left renal tissue. Results showed a significant increase in the levels of MDA, NO, Cr, and BUN and decrease of GSH, CAT, GPx, and SOD by GEN administration. Co-administration with GA showed reduction in the levels of MDA, NO, Cr, and BUN and increase in GSH, CAT, GPx, and SOD. Also, the nephroprotective effect of GA was confirmed by the histological examination of the kidneys. The results of our study showed that GA exerts a significant nephroprotective effect against GEN-induced nephrotoxicity.

KEYWORDS:

Gentamicin; antioxidants; gallic acid; nephrotoxicity; rat

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