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J Exp Ther Oncol. 2017 Sep;11(2):85-89.

Acute effects of low-level laser therapy (660 nm) on oxidative stress levels in diabetic rats with skin wounds.

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Federal University of Mato Grosso do Sul (UFMS), Post Graduate Program in Health and Development of Midwest Region, Campo Grande/MS, Brazil.
Federal University of Piauí (UFPI), Department of Health Sciences, Parnaíba/PI, Brazil.
Federal University of Mato Grosso do Sul (UFMS), Graduate Course of Physiotherapy, Campo Grande/MS, Brazil.
University Anhanguera-UNIDERP, Graduate Course of Veterinary Medicine, Campo Grande/MS, Brazil.
Federal University of Piauí (UFPI), Postgraduate Program in Biomedical Sciences, Parnaíba/PI, Brazil.
University Nove de Julho (UNINOVE), Postgraduate Program in Rehabilitation Sciences and Postgraduate Program in Biophotonics, São Paulo/SP, Brazil.



Laser therapy influences oxidative stress parameters such as the activity of antioxidant enzymes and the production of reactive oxygen species.


To analyze the effects of low-level laser therapy on oxidative stress in diabetics rats with skin wounds.


Thirty-six animals were divided into 4 groups: NDNI: non-diabetic rats with cutaneous wounds that not received laser therapy; NDI: non-diabetic rats with cutaneous wounds that received laser therapy; DNI: diabetic rats with skin wounds who did not undergo laser therapy; DI: rats with diabetes insipidus and cutaneous wounds and received laser therapy. The animals were treated with LLLT (660 nm, 100 mW, 6 J/cm, spot size 0.028 cm). On the day of killing the animals, tissue-wrapped cutaneous wounds were collected and immediately frozen, centrifuged, and stored to analyze malondialdehyde (MDA) levels.


Significant difference was observed within the groups of MDA levels (ANOVA, p = 0.0001). Tukey's post-hoc test showed significantly lower values of MDA in irradiated tissues, both in diabetic and non-diabetic rats. ANOVA of the diabetic group revealed a significant difference (p < 0.01) when all groups, except NDI and DI, were compared.


LLLT was effective in decreasing MDA levels in acute surgical wounds in diabetic rats.


Laser; Malondialdehyde; Oxidative stress

[Indexed for MEDLINE]

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